Reconstitution Fundamentals
Lyophilized peptides are stable powders until you add solvent. The reconstitution step sets the concentration of every dose that follows — get it wrong once and the error carries through every subsequent injection in the protocol.
⚠️ Research Use Only
All products sold by FenaLife are intended strictly for laboratory and academic research purposes. Not for human consumption, injection, or ingestion. These statements have not been evaluated by the FDA.
Solvent Selection by Peptide Type
| Solvent |
When to Use |
| Bacteriostatic Water (BAC) |
Standard choice — 0.9% benzyl alcohol inhibits bacterial growth, extends vial life to 28+ days |
| Sterile Water for Injection |
Single-use protocols only — no preservative, discard within 24 hours |
| 0.9% Sodium Chloride (saline) |
Some peptides with poor water solubility; check individual compound data |
| Dilute Acetic Acid (0.1–1%) |
Certain growth factors and IGF variants; confirm with CoA guidance |
Reconstitution Protocol
1
Draw solvent into syringe
Use a fresh 23G needle for drawing. Pull the exact volume calculated for your target concentration.
2
Insert needle into vial
Pierce the lyophilized vial septum at an angle. Point the needle tip toward the vial wall, not the powder cake.
3
Release solvent slowly
Let it run down the inner wall of the vial. Never inject directly onto the powder — it damages peptide structure.
4
Do not shake
Gently swirl or roll the vial between your palms for 20–30 seconds until fully dissolved. Shaking causes aggregation.
5
Inspect the solution
It should be clear and colorless or faintly yellow. Cloudiness, particles, or unusual color means discard.
6
Label the vial
Date, compound, concentration, and solvent. Refrigerate immediately at 2–8°C.
Concentration Calculations
Every dosing error in peptide research traces back to a miscalculation here. Work through the math before touching the vial.
The Formula
Concentration (mg/mL) = Peptide mass (mg) divided by Solvent volume (mL)
Example: 10mg peptide + 2mL BAC water = 5mg/mL
To draw 500mcg (0.5mg): 0.5 divided by 5 = 0.1mL = 10 units on an insulin syringe
Common Concentration Reference
| Peptide Mass |
Solvent Added |
Concentration |
500mcg dose volume |
| 5mg |
1mL |
5mg/mL |
0.1mL (10 IU) |
| 5mg |
2mL |
2.5mg/mL |
0.2mL (20 IU) |
| 10mg |
2mL |
5mg/mL |
0.1mL (10 IU) |
| 10mg |
5mL |
2mg/mL |
0.25mL (25 IU) |
Dosing Accuracy Factors
Syringe Calibration
Read the meniscus at eye level. Parallax error at an angle adds up across repeated doses.
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Temperature
Draw room-temperature solution. Cold peptide solution is more viscous and draws unevenly.
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Air Bubbles
Flick the syringe and push bubbles out before injecting. Air displaces volume and skews the dose.
Record Keeping
Log every reconstitution: date, lot number, concentration, volume remaining. Catches errors before they compound.
Storage After Reconstitution
Reconstituted Peptide Storage Guidelines
| Storage Condition |
Duration |
| Refrigerated 2–8°C in BAC water |
Up to 28 days |
| Refrigerated 2–8°C in sterile water |
24 hours maximum |
| Frozen at -20°C |
Up to 3 months — avoid repeated freeze-thaw cycles |
| Room temperature |
Not recommended — accelerates degradation |
Freeze-thaw cycles degrade peptide integrity. If a protocol runs longer than 28 days, aliquot the reconstituted solution into single-use volumes before freezing rather than repeatedly thawing the same vial.
Conclusion
Precise reconstitution and accurate dosing calculation are the foundation of reproducible peptide research. FenaLife supplies BAC Water 10mL and BAC Water 3mL alongside its full peptide catalog.
⚠️ Research Use Only
All products sold by FenaLife are intended strictly for laboratory and academic research purposes. Not for human consumption, injection, or ingestion. These statements have not been evaluated by the FDA.
