Humanin Peptide Research Guide: Mitochondrial-Derived Cytoprotective Peptide

Overview

Humanin is a 21-amino acid peptide encoded within the mitochondrial 16S ribosomal RNA gene — making it a mitochondria-derived peptide (MDP). First identified in 2001 while screening for factors protective against Alzheimer’s disease, Humanin has been studied for neuroprotective, cytoprotective, anti-apoptotic, and metabolic effects. Circulating Humanin levels decline with age, and this decline has been associated with increased disease risk, positioning it as a key target in longevity and aging research. FenaLife supplies Humanin for laboratory research use only.

Research Background

Early research established Humanin’s ability to prevent neuronal cell death induced by amyloid beta (Aβ), mutant presenilin-2, and other apoptotic triggers. Research then expanded to cardiomyocytes, pancreatic beta cells, and vascular endothelial cells. Critically, Humanin levels are elevated in centenarians and their offspring compared to age-matched controls. Animal studies using Humanin analogues — particularly HNG (Gly14Ser substitution, ~1000x more potent) — demonstrate protection against stroke, myocardial infarction, and metabolic syndrome in multiple model systems.

Studied Mechanisms

Humanin binds the tripartite receptor complex (CNTFR/WSX-1/gp130), activating JAK2-STAT3 signaling to suppress apoptosis. It also engages FPRL1/FPR2 receptors and interacts with the IGF-1 receptor pathway. Intracellularly, Humanin interacts with IGFBP-3 and BiKE, preventing mitochondrial cytochrome c release and preserving membrane potential. Its mitochondrial origin means it functions as both an autocrine and endocrine signal, detectable in circulation and capable of acting at distant sites.

Molecular Profile

Type: Mitochondria-derived peptide (MDP). Length: 21 amino acids. Molecular weight: ~2887 Da. Key analogue: HNG (Gly14Ser substitution). Encoded in: Mitochondrial 16S rRNA. Purity: ≥98% by HPLC.

Storage and Handling

Store lyophilized Humanin at -20°C or -80°C for long-term preservation. Reconstitute with sterile or bacteriostatic water. Use within 4 to 6 weeks when stored reconstituted at 4°C. Avoid repeated freeze-thaw cycles.

Research Use Only

For laboratory research purposes only. Not for human or veterinary use. Certificate of Analysis available per batch.